Spatial-ATAC-Hi-C maps tissue-wide 3D genome and chromatin accessibility

A new microfluidic Spatial-ATAC-Hi-C platform enables simultaneous, genome-wide mapping of 3D genome architecture and chromatin accessibility directly in tissue sections, preserving spatial context. Applied to mouse and human brains and glioma samples, it reveals cell-type–specific 3D genome features and chromatin loops, detects spatial copy-number variations and structural variants, and uncovers clonal heterogeneity across tumor regions, offering a powerful tool for studying spatial gene regulation in health and disease.