β-catenin mutational spectrum reveals tissue-tuned signaling across cancers

Researchers used saturation genome editing to test all 342 missense mutations in CTNNB1 exon 3, quantifying β-catenin signaling and revealing tissue-specific mutation patterns, a refined β-TRCP docking motif, and a spectrum of activating effects that correlate with hepatocellular carcinoma subtypes and tumor immune infiltration, aiding interpretation of CTNNB1 variants and guiding therapy.