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Shotgun Sequencing

All articles tagged with #shotgun sequencing

Airborne DNA: filters yield a city-to-forest census of life and pathogens
science3 months ago

Airborne DNA: filters yield a city-to-forest census of life and pathogens

A study shows that DNA shed into the air can be captured by standard filters and read with shotgun long‑read sequencing to create a census of organisms in a space, enabling population genetics of wildlife and surveillance for pathogens from samples in Florida and Dublin. The method can identify individual animals from ambient air and detect urban pathogens and plant DNA (e.g., cannabis, poppy, psilocybin mushrooms) in city air, with a two‑day pipeline from filter to analysis using portable equipment. While promising for biodiversity monitoring and early pathogen warning, the approach raises governance and privacy concerns about ambient genetic data and should not be read as evidence of drug use; ethical frameworks are needed before widespread deployment.

Revolutionizing Gut Health: A Breakthrough Method for Analyzing the Human Microbiome
science-and-technology2 years ago

Revolutionizing Gut Health: A Breakthrough Method for Analyzing the Human Microbiome

Researchers at Caltech have developed a new method called microbial enrichment method (MEM) to isolate microbial cells from host cells in the human gut. This method selectively lyses host cells, allowing for a more accurate analysis of the gut microbiome. MEM performed well in comparison to other techniques and enabled high-throughput metagenomic characterization from host-rich samples. The use of MEM followed by shotgun sequencing could provide a deeper understanding of the gut microbiome's role in human health and disease.