"Fluoropolymer Nanosheet Enables Large-Scale In Vivo Mouse Brain Imaging"

A new method utilizing a biocompatible nanosheet incorporated into light-curable resin (NIRE method) has been proposed to construct large cranial windows over the cortex and cerebellum suitable for long-term imaging in awake mice. The NIRE method produced windows that maintained transparency for over 5 months and suppressed motion artifacts, enabling the visualization of neural structures and intracellular Ca2+ concentration changes at various scales, from populations of over a thousand neurons to single spines, in living mouse brain. This method allows for multi-scale in vivo imaging of neuronal morphology and intracellular calcium in awake mice, demonstrating its potential for studying brain function and pathology.