All articles tagged with #two photon microscopy
Using intravital two-photon microscopy, researchers watched CD169-expressing macrophages in the skin’s hypodermis engulf live melanoma cells without T or B cells, a finding that depletion of these macrophages worsened tumor growth and that similar macrophage populations were found at human melanoma margins—suggesting a way to turn ‘cold’ tumors ‘hot’ and possibly enhance responses to immune checkpoint inhibitors.
A new method utilizing a biocompatible nanosheet incorporated into light-curable resin (NIRE method) has been proposed to construct large cranial windows over the cortex and cerebellum suitable for long-term imaging in awake mice. The NIRE method produced windows that maintained transparency for over 5 months and suppressed motion artifacts, enabling the visualization of neural structures and intracellular Ca2+ concentration changes at various scales, from populations of over a thousand neurons to single spines, in living mouse brain. This method allows for multi-scale in vivo imaging of neuronal morphology and intracellular calcium in awake mice, demonstrating its potential for studying brain function and pathology.
Researchers conducted experiments on mice to investigate minute-scale oscillatory sequences in the medial entorhinal cortex (MEC). They performed surgeries to implant microprisms or glass windows for optical access to the MEC and adjacent areas. Two-photon calcium imaging and electrophysiological data were collected, and Neuropixels probes were used for recording neural signals. The mice were trained to run on a wheel under minimal sensory stimulation conditions. The study provides insights into the neural activity patterns in the MEC and their potential role in spatial navigation and memory.