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Two Photon Microscopy

All articles tagged with #two photon microscopy

science24 days ago•6 min saved

Astrocyte Nuclei Travel to Rebuild Damaged Brain Networks

Researchers at the University of Zurich identified a novel regenerative mechanism in the adult brain: specialized perilesional astrocytes do not rely solely on local division but instead generate nuclei that migrate along long astrocytic extensions into the injury core, repopulating damaged tissue and rebuilding functional networks. Using in vivo two-photon microscopy and longitudinal gene mapping in mice, they mapped activated genes and signaling pathways that could be therapeutic targets to enhance repair after traumatic brain injury and autoimmune conditions like NMOSD. This challenges the long-held view of limited glial regeneration and suggests new avenues for brain repair therapies.

via Neuroscience News|

#astrocytes #brain-regeneration #nmosd

science3 months ago•10 min saved

Real-time imaging reveals macrophages devouring live melanoma cells

Using intravital two-photon microscopy, researchers watched CD169-expressing macrophages in the skin’s hypodermis engulf live melanoma cells without T or B cells, a finding that depletion of these macrophages worsened tumor growth and that similar macrophage populations were found at human melanoma margins—suggesting a way to turn ‘cold’ tumors ‘hot’ and possibly enhance responses to immune checkpoint inhibitors.

via The Conversation|

#cd169 #immunotherapy #macrophages

science-and-technology2 years ago•49 min saved

"Fluoropolymer Nanosheet Enables Large-Scale In Vivo Mouse Brain Imaging"

A new method utilizing a biocompatible nanosheet incorporated into light-curable resin (NIRE method) has been proposed to construct large cranial windows over the cortex and cerebellum suitable for long-term imaging in awake mice. The NIRE method produced windows that maintained transparency for over 5 months and suppressed motion artifacts, enabling the visualization of neural structures and intracellular Ca2+ concentration changes at various scales, from populations of over a thousand neurons to single spines, in living mouse brain. This method allows for multi-scale in vivo imaging of neuronal morphology and intracellular calcium in awake mice, demonstrating its potential for studying brain function and pathology.

via Nature.com|

#cranial-window #in-vivo-imaging #mouse-brain

neuroscience2 years ago•86 min saved

"Neural Rhythms: Unveiling the Brain's Orderly Patterns"

Researchers conducted experiments on mice to investigate minute-scale oscillatory sequences in the medial entorhinal cortex (MEC). They performed surgeries to implant microprisms or glass windows for optical access to the MEC and adjacent areas. Two-photon calcium imaging and electrophysiological data were collected, and Neuropixels probes were used for recording neural signals. The mice were trained to run on a wheel under minimal sensory stimulation conditions. The study provides insights into the neural activity patterns in the MEC and their potential role in spatial navigation and memory.

via Nature.com|

#calcium-imaging #electrophysiology #medial-entorhinal-cortex