Tag

Fluorescent Imaging

All articles tagged with #fluorescent imaging

science17 days ago•4 min saved

AI-Designed NovoTags Expand Multicolor Live-Cell Imaging

Researchers at HHMI’s AI@HHMI, led by David Baker and Luke Lavis, are using RFdiffusion-based AI to design NovoTags—small protein binders that pair with Janelia Fluor dyes—to create a new class of fluorescent probes. This approach could let scientists label many colors and proteins simultaneously, without traditional chemical linkers, enabling longer, multi-color imaging and accelerating discovery. The team plans to rollout NovoTags for about a dozen dye colors and expand to dyes that blink or respond to physiological signals, with the tools becoming broadly available to the scientific community.

via HHMI|

#ai #fluorescent-imaging #novotag

science-and-technology2 years ago•5 min saved

Revolutionary Imaging Technique Reveals Intricate Inner Workings of Living Cells

MIT researchers have developed a new method for imaging living cells that allows them to observe up to seven different molecules at a time, potentially enabling a comprehensive study of cellular behavior. The technique involves using switchable fluorophores, fluorescent proteins that turn on and off at different rates, to label different types of molecules within a cell. By imaging the cell over time and using a computational algorithm to extract each fluorescent signal, researchers can track changes in the amount of each target protein. This approach could provide insights into various biological phenomena, including aging, disease, and cellular responses to different stimuli.

via MIT News|

#biological-phenomena #cell-biology #computational-algorithm