TNBC’s heterogeneity mapped: four cancer archetypes and eight tissue ecotypes forecast chemo response

A large single‑cell and spatial transcriptomics study of pretreatment TNBC identifies four cancer-cell archetypes and eight ecotypes defined by cancer cells and the tumor microenvironment, reveals macrophage and interferon‑related programs linked to better neoadjuvant chemotherapy response, and provides cell-state– and gene-based classifiers (including a 13‑gene panel) to predict pathological complete response, offering new insight into TNBC heterogeneity and potential immune targets such as LAG3 and HAVCR2.