Ancient DNA nails malaria as the Medici brothers’ demise

An international team analyzed the 16th‑century remains of Cardinal Giovanni de’ Medici and Grand Duke Francesco I and found DNA evidence of malaria parasites, confirming they died of malaria rather than poisoning; Francesco’s bones show infection with both P. falciparum and P. malariae, while Giovanni carried a novel P. falciparum strain. The work, published in iScience, provides insight into malaria’s evolution in Europe and suggests swampy Tuscany environments facilitated transmission in the Medici era.