PCA-iSIM: A Compact, Fast Route to Real-Time Super-Resolution Microscopy

Researchers from the Smart Computational Imaging Laboratory (SCILab) at Nanjing University of Science and Technology unveil PCA-iSIM, a compact DMD-based incoherent structured-illumination microscopy framework that couples high-modulation coefficient mapping with principal component analysis to recover high-frequency details from low-contrast patterns, enabling real-time imaging at up to 30 frames per second with about 100 nm resolution and a ~70% simpler optical design than laser-based SIM; demonstrated in living cells imaging mitochondrial activity under challenging noise and setting changes.