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Gfp

All articles tagged with #gfp

science17 days ago•6 min saved

Big Expansion Enables Simple Microscopes to See Amino Acids in Proteins

Researchers boosted sample size up to 1,000-fold per dimension with a new hydrogel recipe and ONE microscopy, allowing ordinary light microscopes to pinpoint amino acids within proteins (including GFP and a nanobody) and map protein structures at near-nanometer detail. While still short of cryo-EM/X-ray resolution, the approach could democratize high-resolution structural biology by making detailed molecular layouts accessible with standard fluorescence microscopes.

via Nature|

#amino-acids #expansion-microscopy #gfp

science-and-technology2 years ago•6 min saved

"Revolutionizing Protein Engineering with Computational Techniques and AI Integration"

MIT researchers have developed a computational approach to predict mutations that will lead to improved proteins, making it easier to engineer proteins with useful functions. Using a convolutional neural network trained on experimental data, the researchers created fitness landscapes to predict optimized versions of green fluorescent protein (GFP) and a protein from adeno-associated virus (AAV). The approach has the potential to accelerate the process of optimizing proteins for research and medical applications, and could be applied to other protein engineering problems.

via MIT News|

#aav #computational-technique #gfp

science-and-technology2 years ago•2 min saved

"Rapid Fingerprint Detection with Jellyfish Protein Spray"

Scientists have developed a forensic spray using a protein found in jellyfish that can reveal fingerprints in just 10 seconds, making forensic investigations quicker and more effective. The water-soluble and low-toxicity dye spray, based on a fluorescent compound called green fluorescent protein (GFP), is biologically compatible and does not interfere with DNA analysis. The spray comes in two colors, binds to chemicals in sweat and oils, and emits a fluorescent glow under blue light. This innovation offers a safer, more sustainable, and faster alternative to traditional fingerprint detection methods, and the team aims to make the forensic dyes commercially available.

via The Guardian|

#fingerprint-detection #forensic-investigation #forensic-science