Tag

Recombinases

All articles tagged with #recombinases

Mobilome drives microbial diversity and function in Arctic thaw soils
science15 days ago

Mobilome drives microbial diversity and function in Arctic thaw soils

An eight-year meta-omic study of Stordalen Mire soils reveals ~2.1 million mobile genetic element (MGE) recombinases across 89 microbial phyla, with insertion sequences/transposons dominating and an active mobilome reshaping host gene content and metabolic functions—especially those related to carbon flux and nutrient cycling—across palsa, bog and fen habitats. By comparing short- and long-read assemblies, the work quantifies MGE recovery and mobility, provides metatranscriptomic/metagenomic metrics of MGE activity, and offers a framework to quantify MGEs’ ecological impacts in complex natural microbiomes during permafrost thaw.

"Genome Engineering Tool: Beyond CRISPR"
science-and-technology2 years ago

"Genome Engineering Tool: Beyond CRISPR"

Belgian researchers have developed a new toolbox of 16 short DNA sequences that enable controlled and specific recombination events in any genome, complementing and surpassing CRISPR for certain applications. This toolbox, reported in two concurrent papers in Nature Communications, utilizes site-specific recombinases to address the shortcomings of CRISPR, offering a solution to issues such as toxicity of DNA double strand breaks, low editing efficiencies, and complex patenting. The toolbox has been demonstrated to efficiently optimize gene expression in microbial cell factories and offers possibilities for scientists and industry working in genome engineering.