All articles tagged with #embryogenesis
A mouse oocyte cytoplasmic lattice (CPL) structure was resolved by cryo-EM, revealing a repeating filament composed of U-shaped baskets and adapter rings built from 14 protein subunits; PADI6 anchors the baskets, while SCMC dimers connect adjacent units, outlining how CPL assembles and supporting its crucial roles in oocyte maturation, early embryogenesis, and potential female reproductive disorders.
Using Pico-C, a low-input Micro-C method, the study maps high-resolution 3D genome architecture across NC9–NC14 in early Drosophila embryos and reveals that chromatin loops and boundaries emerge in an ordered, modular fashion before major ZGA. Transcriptional elongation inhibition preserves some early loops but weakens promoter insulation, while sequence-based models show orthogonal motif-driven contributions to architecture. Co-depleting Zelda and GAF confirms locus-specific regulatory inputs, supporting a layered regulatory logic for genome establishment prior to ZGA.
The article introduces spatial-DMT, a novel technology for simultaneous spatial profiling of DNA methylome and transcriptome in tissue sections at near single-cell resolution, applied to mouse embryos and brains, revealing intricate gene regulation mechanisms during development and tissue-specific epigenetic landscapes.
Researchers have successfully cultivated primate embryos in a lab for 25 days post-fertilization, achieving key developmental landmarks never before observed in culture, including the start of organ development. This breakthrough could help understand congenital birth defects and organ development in humans. The embryos surviving to 25 days had many of the hallmarks of an in utero embryo, such as the three embryonic tissue layers ultimately responsible for the formation of the fetus and the formation of the neural tube, which goes on to form the brain and spinal cord.